Effect of Different Levels of Glycerol and DMSO on Freezability of Buck Semen
نویسندگان
چکیده
Accepted: 21/07/2015 Abstract A total 196 ejaculates from 8 adult Sirohi bucks were utilized to find out the freezability of buck semen at different levels of two cryoprotectants. The ejaculates were extended to maintain sperms concentration approximately 100 million per dose (0.25 ml) containing TrisCitric acidFructose (TCF) with different concentrations of glycerol and DMSO viz. 4%, 6% and 8% (v/v) as a cryo-protecting agent. Filling and sealing of straws were done at 5oC in cold handing cabinet after 4 hr of equilibration period, then straws were vapor frozen for 10 minutes above 2 cm of liquid nitrogen and finally put in to liquid nitrogen. Post thaw motility, live sperm count, acrosomal integrity was conducted to know freezability. The results showed that the 6% Glycerol level significantly improved sperm post thaw progressive motility.
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